Test #	TL49SK0054
First Edition	March 31st 2021

Test Implementation Results Report

Effect Verification Test on the Impact of Wearing Relive Shirts and Pants on the Body

March 31st, 2021

Company: ＴＦＣ Lab

 

1. Test Design: This test was an open trial that evaluated grip strength, back muscle strength, range of motion in the neck, shoulders, and lower back, and fatigue levels before wearing the "Relive Shirt and Pants," immediately after wearing them, and one week after wearing them.

2. Selection and Exclusion Criteria for Subjects

(1) Target Group: The study targeted 15 healthy Japanese women between the ages of 30 and 49 who met the selection criteria for this trial, voluntarily agreed to participate as subjects, received a thorough explanation of the trial's content, and provided written consent to participate.

(2) Selection Criteria: 

- Age: 30 years or older, but under 59 years (at the time of consent)

- Gender: Female

1. Healthy individuals (In this trial, a healthy individual is defined as someone without severe organ damage or specific diseases, who is not receiving related treatments, and who is not undergoing any form of pharmacotherapy. Whether an individual qualifies as healthy will be based on their self-report at the start of the trial.)

2. Individuals engaged in activities that require physical strength on a regular basis.

3. Individuals who experience fatigue and discomfort or pain in the neck, shoulders, and lower back as a result of the above activities.

4. Individuals who do not regularly visit chiropractic clinics or sports gyms.

5. Individuals not currently participating in other trials.

6. Individuals who can provide written consent.

7. Individuals who do not fall under the following exclusion criteria for trial participants.

（３） Exclusion Criteria

1. Individuals with a history of treatment for heart failure, myocardial infarction, or similar conditions.

2. Exclusion due to specific diseases (including atrial fibrillation, arrhythmias, liver disorders, kidney disorders, cerebrovascular disorders, rheumatoid arthritis, diabetes, dyslipidemia, hypertension, and other chronic conditions under treatment), or individuals who regularly attend medical appointments and use medications (including traditional Chinese medicine).

3. Individuals who regularly attend medical appointments and use medications (including traditional Chinese medicine).

4. Individuals who are pregnant, breastfeeding, or may become pregnant during the trial period.

5. Individuals who do not engage in activities that require physical strength on a regular basis.

6. Individuals who do not experience fatigue or discomfort in the neck, shoulders, or lower back.

7. Individuals who regularly visit chiropractic clinics or sports gyms.

8. Individuals who have participated in another clinical trial within the past month or are currently participating in one.

9. Other individuals deemed unsuitable as subjects for this trial by the principal investigator.

（４）Ethics Committee Review and Participant Consent
This trial was conducted in accordance with the spirit of the Declaration of Helsinki, following approval from the Ethics Review Committee of Koizumi Dermatology Clinic (Chairperson: Makoto Koizumi) on January 27, 2021. The purpose and methods of the trial were thoroughly explained to the participants, and written consent was obtained before the trial was conducted.

3. Test Subject
   The test products used in this trial, the "Relive Shirt and Pants," are made from 100% polyester, a stretchable material designed to stimulate muscles and align meridians. Power stones are blended into the dye and incorporated into the fabric at the shoulders, abdomen, thighs, and buttocks.

4. Testing Institution
The test was conducted by TFC Lab Co., Ltd., and the testing took place in the measurement room at the TFC Lab facility.

5. Test Schedule

The test schedule was as follows: measurements before wearing were conducted over two days, February 17 and 18, 2021; measurements immediately after wearing were conducted over two days, February 24 and 25; and measurements one week after wearing were conducted over two days, March 3 and 4. The test schedule is outlined in Table 1.

【Table 1: Test Schedule】

	Before the Test	Before Wearing	Immediately After Wearing	After Wearing  For 1 Week	Follow-up Observation
Screening	〇
Explanation & Obtaining Consent		〇
Subjective Questionnaire		〇	〇	〇
Grip Strength Measurement  (Left & Right)		〇	〇	〇
Back Muscle Strength Measurement		〇	〇	〇
Fatigue Level Measurement		〇	〇	〇
Body Balance Measurement		〇	〇	〇
Range of Motion Measurement		〇	〇	〇
Adverse Effects		〇	〇	〇	〇

＊The follow-up observation period was set at 2 weeks.

6. Test Method

Measurements were taken while all participants wore the same long-sleeve T-shirt and pants without wearing the "Relive Shirt and Pants." Immediately after wearing, all participants wore the "Relive Shirt and Pants," with the shirt worn over the long-sleeve T-shirt and the pants worn over their underwear, and measurements were taken. One week after wearing, participants were required to wear the "Relive Shirt and Pants" for at least 8 hours a day for 7 days, and then measurements were taken.

7. Test Content  

The trial was conducted with 15 participants who met the selection criteria from among those who expressed a desire to participate. Participants who joined the trial completed a questionnaire and measurements before wearing the test items after receiving an explanation of the trial and providing consent. The objective evaluations included the following: neck range of motion was measured; shoulder range of motion was assessed by taking photographs while the participant raised their hands horizontally from a standing position; waist range of motion was measured by having the participant bend forward. Grip strength was measured for both hands using a digital grip strength meter (N-FORCE). Back muscle strength was measured using a digital back strength meter (Back D TKK5402). Fatigue was assessed using the FLICKER fatigue measurement device. The box lifting test involved lifting an 18.9 kg box vertically from a table, and the distance from the floor to the bottom of the box was measured with a tape measure. Subjective evaluations were conducted by having participants rate themselves on seven items: "Range of motion in the arms," "Range of motion in the waist," "Fatigue," "Stress," "Ability to hold objects smoothly," "Quality of sleep," and "Waking up refreshed in the morning."

8.  During the Test
For movements involving raising the arms or bending the waist, there is a possibility that the range of motion may increase on the second attempt if performed within a short period. Therefore, only one measurement was taken. Additionally, participants were instructed not to force the stretch and to stop at the point where they felt resistance (discomfort) or pain.

9. Evaluation Method
   The evaluation method involved a comparative analysis over time, comparing the results before wearing, immediately after wearing, and one week after wearing.

Objective Evaluations:

1. Neck Range of Motion: The angle at which the neck was moved horizontally to the side without moving the shoulders was measured using a digital angle meter, with measurements taken for both left and right sides.

2. Shoulder Range of Motion: From a standing position, participants raised their arms horizontally, and the angle was measured using image analysis from photographs.

3. Waist Range of Motion: Participants bent forward, and the distance between the floor and the middle finger of the right hand was measured using a tape measure.

4. Grip Strength: Grip strength for both hands was measured using a digital grip strength meter (N-FORCE).

5. Back Muscle Strength: Back muscle strength was measured using a digital back strength meter (Back D TKK5402).

6. Fatigue Level: Fatigue was measured using the FLICKER fatigue measurement device, which counted down from 60 until a red dot was observed.

7. Box Lifting Test: Participants lifted an 18.9 kg box vertically from a table, and the distance from the floor to the bottom of the box was measured using a tape measure.

Subjective Evaluations: Participants were asked to self-evaluate on seven items:

1. "Range of motion in the arms" and "Range of motion in the waist" were evaluated on a 9-point scale, ranging from "1 point: difficult to move" to "9 points: easy to move."

2. "Fatigue" and "Stress" were evaluated on a 9-point scale, ranging from "1 point: feeling it" to "9 points: not feeling it."

3. "Ability to hold objects smoothly," "Quality of sleep," and "Waking up refreshed in the morning" were evaluated on a 9-point scale, ranging from "1 point: not feeling it" to "9 points: feeling it."

10. Statistical Method
Each data point was represented using descriptive statistics (mean, standard deviation), and a time-based comparison before and after the intervention was conducted using the Paired t-test. The analysis was performed using Statcel4 software, with a significance level set at 5%

11. Conflict of Interest
There are no conflicts of interest between the joint research institutions, contracting companies, and implementing agencies involved in this trial.

12. Results
All 15 participants completed the trial (mean age 39.9±4.7 years). There were no adverse events, and no participants were deemed ineligible, so all 15 participants were included in the analysis.

1. Changes in Objective Indicators (Table 2, Graph 1, Image 2)

- Neck Range of Motion:

  - Left: Increased from 61.57° before wearing to 79.08° immediately after wearing, and to 87.45° one week after wearing.

  - Right: Increased from 50.42° before wearing to 69.38° immediately after wearing, and to 78.20° one week after wearing.

  

- Arm Range of Motion:

  - Left: Increased from 99.93° before wearing to 159.00° immediately after wearing, and to 173.13° one week after wearing.

  - Right: Increased from 97.40° before wearing to 159.07° immediately after wearing, and to 174.13° one week after wearing.

- Forward Bending (Flexion): 

  Improved from 10.43 cm before wearing to 4.47 cm immediately after wearing, and to -1.87 cm one week after wearing.

- Grip Strength:

  - Left: Increased from 22.01 kg before wearing to 25.91 kg immediately after wearing, and to 27.42 kg one week after wearing.

  - Right: Increased from 24.75 kg before wearing to 27.59 kg immediately after wearing, and to 28.40 kg one week after wearing.

- Back Muscle Strength: 

  Increased from 48.27 kg before wearing to 66.57 kg immediately after wearing, and to 74.68 kg one week after wearing.

- Fatigue Level:  

  Improved from 33.47 before wearing to 34.50 immediately after wearing, and to 37.73 one week after wearing.

- Box Lifting:  

  Improved from 0.81 cm before wearing to 4.53 cm immediately after wearing, and to 10.93 cm one week after wearing.

【Table 2: Changes in Objective Indicators】

Category	Measured Value			Amount of Change		Time-based Change: p-value
	Before Wearing	Immediately After Wearing	After Wearing for  1 Week	Before Wearing / Immediately After Wearing	Before Wearing /  After Wearing for 1 Week	Before Wearing / Immediately After Wearing	Before Wearing - One Week After Wearing
Left Neck Range of Motion：°	61.57 ± 17.46	79.08 ± 13.69	87.45 ± 17.25	17.51 ± 10.77	25.88 ± 13.65	<0.01 **	<0.01 **
Right Neck Range of Motion：°	50.42 ± 11.88	69.38 ± 13.31	78.20 ± 15.05	18.96 ± 9.65	27.78 ± 14.23	<0.01 **	<0.01 **
Left Arm Range of Motion: °	99.93 ± 21.32	159.00 ± 23.24	173.13 ± 14.35	59.07 ± 29.60	73.20 ± 25.44	<0.01 **	<0.01 **
Right Arm Range of Motion: °	97.40 ± 19.82	159.07 ± 23.70	174.13 ± 13.56	61.67 ± 29.64	76.73 ± 24.41	<0.01 **	<0.01 **
Forward Bend：cm	10.43 ± 9.17	4.47 ± 6.95	-1.87 ± 11.12	-5.97 ± 3.91	-12.30 ± 11.12	<0.01 **	<0.01 **
Left Grip Strength：kg	22.01 ± 6.37	25.91 ± 4.82	27.42 ± 4.44	3.91 ± 3.04	5.41 ± 3.85	<0.01 **	<0.01 **
Right Grip Strength：kg	24.75 ± 6.01	27.59 ± 5.03	28.40 ± 4.15	2.84 ± 3.18	3.65 ± 3.56	<0.01 **	<0.01 **
Back Muscle Strength：kg	48.27 ± 24.55	66.57 ± 21.09	74.68 ± 18.53	18.30 ± 14.06	26.41 ± 18.87	<0.01 **	<0.01 **
Fatigue Level: Index	33.47 ± 2.33	34.50 ± 3.72	37.73 ± 5.26	1.03 ± 2.55	4.27 ± 4.17	0.14 n.s.	<0.01 **
Box Lifting: cm	0.81 ± 0.70	4.53 ± 3.60	10.93 ± 6.19	3.73 ± 3.17	10.12 ± 5.68	<0.01 **	<0.01 **

**Mean ± Standard Deviation, n.s.: p > 0.05, *: p ≤ 0.05, : p < 0.01

【Graph 1: Changes in Objective Indicators】(Before Wearing, Immediately After Wearing, After Wearing for 1 Week)

【Image 2: Range of Motion of the Arm, Surface Body Temperature - Before and After】

 

2. Changes in Subjective Indicators (Table 3, Graphs 2 and 3)
   Regarding the range of motion of the arm, the score was 3.16 points before wearing, 6.40 points immediately after wearing, and 8.47 points one week after wearing.
   As for the range of motion of the lower back, the score was 2.80 points before wearing, 5.60 points immediately after wearing, and 7.93 points one week after wearing.
   In terms of fatigue, the score was 2.13 points before wearing, 5.00 points immediately after wearing, and 7.73 points one week after wearing.
   Regarding stress, the score was 3.13 points before wearing, 5.40 points immediately after wearing, and 7.53 points one week after wearing.
   For ease of holding objects, the score was 3.60 points before wearing, 6.80 points immediately after wearing, and 8.07 points one week after wearing.
   As for getting sufficient sleep, the score was 3.13 points before wearing, 4.67 points immediately after wearing, and 7.47 points one week after wearing.
   Regarding waking up refreshed in the morning, the score was 2.53 points before wearing, 4.40 points immediately after wearing, and 7.87 points one week after wearing.

【Table 3: Changes in Subjective Indicators】

Category	Measured Value			Amount of Change		Time-based Change: p-value
	Before Wearing	Immediately After Wearing	After Wearing for 1 Week	Before Wearing / Immediately After Wearing	Before Wearing / After Wearing for 1 Week	Before Wearing / Immediately After Wearing	Before Wearing - One Week After Wearing
Arm Range of Motion (Points)	3.13 ± 1.19	6.40 ± 1.45	8.47 ± 0.74	3.27 ± 1.75	5.33 ± 1.45	<0.01 **	<0.01 **
Lower Back Range of Motion (Points)	2.80 ± 1.08	5.60 ± 1.55	7.93 ± 1.28	2.80 ± 2.01	5.13 ± 1.85	<0.01 **	<0.01 **
Regarding Fatigue (Points)	2.13 ± 1.19	5.00 ± 1.69	7.73 ± 1.28	2.87 ± 2.13	5.60 ± 2.06	<0.01 **	<0.01 **
Regarding Stress (Points)	3.13 ± 1.13	5.40 ± 1.72	7.53 ± 1.25	2.27 ± 1.87	4.40 ± 1.72	<0.01 **	<0.01 **
Ease of Holding Objects (Points)	3.60 ± 1.35	6.80 ± 1.21	8.07 ± 0.80	3.20 ± 1.78	4.47 ± 0.80	<0.01 **	<0.01 **
Getting Sufficient Sleep (Points)	3.13 ± 0.74	4.67 ± 1.40	7.47 ± 1.36	1.53 ± 1.41	4.33 ± 1.50	<0.01 **	<0.01 **
Wake Up Feeling Refreshed (Points)	2.53 ± 0.99	4.40 ± 1.55	7.87 ± 1.30	1.87 ± 1.73	5.33 ± 2.02	<0.01 **	<0.01 **

Mean ± Standard Deviation, n.s.: p > 0.05, *: p ≦ 0.05, **: p < 0.01

(Before Wearing, Immediately After Wearing, After Wearing for 1 Week)

【Graph 3: Changes in Subjective Indicators】

 

 

【Graph 4: Experience Survey】(Felt the Effects/Did Not Feel)

 

13. Discussion
This study evaluated the effects of wearing "Relive Shirt & Pants" over a period of two weeks. The "Relive Shirt & Pants" are designed to stimulate meridians and muscles through negative ions and other elements emitted from ores mixed into the fabric pigment. This, in turn, helps regulate the flow of electrical signals from the brain, contributing to overall body alignment.

The range of motion improvements observed were as follows:

• For the left neck, the range of motion increased by 28.4% immediately after wearing and by 42.0% after one week.

• For the right neck, it increased by 37.6% immediately after wearing and by 55.1% after one week.

• For the left arm, the range of motion increased by 59.1% immediately after wearing and by 73.2% after one week.

• For the right arm, it increased by 63.3% immediately after wearing and by 78.8% after one week.

• For forward bending (flexion), the range of motion increased by 57.2% immediately after wearing and by 117.9% after one week.

These results suggest that wearing the "Relive Shirt & Pants" leads to an immediate improvement in range of motion, with even greater effects observed after one week of use.

Regarding the impact on muscle strength:

• Grip strength increased by 17.8% in the left hand immediately after wearing and by 24.6% after one week.

• In the right hand, grip strength increased by 11.5% immediately after wearing and by 14.8% after one week.

• Back muscle strength increased by 37.9% immediately after wearing and by 54.7% after one week.

In a test where a box (18.9 kg) was lifted horizontally from a table to see how high it could be lifted:

• Before wearing, the box was lifted 0.81 cm,

• Immediately after wearing, it was lifted 3.73 cm,

• And after one week, it was lifted 10.12 cm.
  Although the box could hardly be lifted before wearing the garments, it was lifted significantly after wearing them.

In terms of fatigue levels, no significant difference was observed immediately after wearing, but a significant difference was noted after one week (p < 0.01**). In self-reported questionnaires, participants indicated a reduction in shoulder and lower back stiffness and pain, and they no longer felt daily fatigue carrying over to the next day.

These findings suggest that wearing the "Relive Shirt & Pants" stimulates meridians and muscles, improves core stability, enhances body movement and strength, and improves blood circulation, which helps reduce stiffness and pain.

14. Conclusion
Wearing the "Relive Shirt & Pants" suggests improvements in range of motion, grip strength, and back muscle strength, as well as a reduction in daily fatigue. Among the 15 participants in this study, no one reported any discomfort during the 2-week observation period, indicating that the "Relive Shirt & Pants" are safe to use.